Resultados

ABSTRACT

INTRODUCTION: Enzymes are protein molecules that are able to facilitate and accelerate the chemical reactions that occur in vivo. The rate of enzymatic reaction may vary depending on the enzyme concentration, substrate concentration, temperature, pH and inhibitors which reduce the rate of reaction by attaching to the enzyme. Catecholase is a oxireductasa the enzyme that catalyzes the oxidation of some organic compounds using oxygen as a hydrogen receptor. OBJECTIVE: To identify the reaction that catalyzes the catecholase and observe enzymatic activity with the reaction when subjected to different factors. METHODOLOGY: 5 tests were conducted for the practice consisted which make a variation of substances with the use of catechol, enzyme extract, distilled water, phenol, hydroquinone, lead nitrate, and ascorbic acid and subjected to different temperature and pH; so have a variation of results and colorations. RESULTS: in the realization of tables and graphs showing changes enzyme activity staining were observed by substrate used and the temperature at which is exposed giving as a result different colors in red, pink, white and brown. Showing the reaction of the enzyme on the substrate. CONCLUSION: the basics of enzymes, as well as their functions and in the laboratory have a good handle on these and observe the processes and reactions that such conduct is recognized. Identifying its catalytic action.

KEYWORDS: enzyme, catechol, enzymatic activity, substrate, catecholase

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